Selective autophagy in proteostasis maintenance 

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Building on expertise and methods established in the 1st funding period, we seek to (i) identify selective autophagy receptor-specific cargo in fed and proteostasis-challenged cells using quantitative proteomics-based autophagosome content profiling, (ii) determine the molecular grammar and machinery that drives autophagic cargo engagement under these conditions, and (iii) develop a LC3B-based reporter in mice to examine physiologically relevant autophagy cargo alterations upon proteostasis challenges in a cell type-specific manner in these animals. Overall, this project will substantially advance our understanding of proteostasis maintenance by selective autophagy pathways.

 

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