regulatory mechanisms and dynamics of er-phagy receptor clustering

Receptors involved in the selective autophagy of the endoplasmic reticulum (ER-phagy) are paramount in the remodeling of the ERnetwork. However, their regulation and spatial organization on the nanoscale under basal and stress conditions are still elusive. The introduction of novel non-covalent low affinity fluorescent labels for super-resolution microscopy and application of deep neural-networks facilitates multi-target, high-density and high-throughput imaging with near-molecular resolution. Using these concepts in the context of ER-phagy allows us to visualize the spatial organization and interactome of ER-phagy receptors, thus providing insights into the orchestration and molecular function of key regulators of ER-phagy.

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