F06
regulatory mechanisms and dynamics of er-phagy receptor clustering
Receptors involved in the selective autophagy of the endoplasmic reticulum (ER-phagy) are paramount in the remodeling of the ERnetwork. However, their regulation and spatial organization on the nanoscale under basal and stress conditions are still elusive. The introduction of novel non-covalent low affinity fluorescent labels for super-resolution microscopy and application of deep neural-networks facilitates multi-target, high-density and high-throughput imaging with near-molecular resolution. Using these concepts in the context of ER-phagy allows us to visualize the spatial organization and interactome of ER-phagy receptors, thus providing insights into the orchestration and molecular function of key regulators of ER-phagy.
prof. dr. mike heilemann
Institute for Physical and Theoretical Chemistry
GU Frankfurt
Max-von-Laue-Str. 7
60438 Frankfurt a. M
Germany
Office: +49 69 798 29736
heilemann@chemie.uni-frankfurt.de
DR. alexandra stolz
Institute of Biochemistry II
GU Frankfurt
Theodor Stern Kai 7
60590 Frankfurt a. M.
Germany
Office: +49 69 798 42589
stolz@em.uni-frankfurt.de